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DNA to proteins
Major concepts:
- Molecular biology as a discipline in Biology has
grown enormously in the last 50 years. In the beginning, molecular biologists
wanted to understand the structure of biological molecules (proteins
and DNA for example). More recently they have focused on a variety of
issues about those molecules. Most importantly, they wanted to understand
how genetic information in the form of the nucleic acid DNA is expressed
in a useful form in cells.
- In the 1950s it became clear that DNA contained
the information necessary for construction of proteins. Early in the
1960s molecular biologists found evidence of an unstable form of nucleic
acid, ribonucleic acid or RNA, that had the properties of a messenger.
Messenger RNA (mRNA) is an exact copy of a short section of the DNA
and carries that information to where proteins are being constructed.
In a eukaryote DNA resides in the nucleus and proteins are constructed
in the cytoplasm, so mRNAs must travel out of the nucleus into the cytoplasm.
- The central organizing principle of molecular biology,
called with tongue somewhat in cheek the "Central Dogma",
is that DNA is expressed into RNA which is expressed into protein. More
succinctly: DNA®RNA®protein.
- The sequence of monomers in a DNA polymer (nucleotides)
must first be copied into an exact replica in RNA. Using the analogy
of a scribe copying out ("transcribing") a manuscript, this
process is termed transcription. Then the mRNA must be converted from
the "language" of nucleic acids (nucleotides) into the language
of proteins (which are polymers of amino acids). Synthesis of proteins
following mRNA instructions is called translation.
- The one-to-one translation of proteins from mRNA
relies on a code, the "genetic code", in which a group of
three adjacent nucleotides (a "codon") specifies one of the
20 amino acids found in proteins. In the process of translation each
successive codon in an mRNA is decoded into an amino acid, with the
amino acids being joined together into a protein polymer. The protein
product, when finished and released, folds spontaneously into a structure
that allows it to perform its cellular function.
Pages in this unit:
- The "Central
Dogma" expresses how the information in DNA becomes converted into
proteins.
- DNA copied into an
exact replica as an mRNA in a process called "transcription"
- The process of converting
an mRNA sequence into a protein is called translation
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