This image was taken at f#22 as an exercise in depth of field.  The scan of the square slide was cropped to allow for a video aspect ratio of 3:4 for LCSG full frame viewing.  The vignetted corners were also removed.  An enlarged view of the scan is available by clicking on the image.  You will notice that best sharpness is at ~ 4 ft and that sharpness in the distance has been sacrificed.  The smallest focused spot of 500nm green would be 26 microns, or 40 dots per mm (20 lp/mm).  Since the Fuji Chrome can resolve 50 lp/mm there is some overall loss of image sharpness in spite of the increased depth of field.  These changes in resolution are not highly visible in this jpg.