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DNA Repair

Importance

DNA in the living cell is subject to many chemical alterations (a fact often forgotten in the excitement of being able to do DNA sequencing on dried and/or frozen specimens [Link]). If the genetic information encoded in the DNA is to remain uncorrupted, any chemical changes must be corrected.

A failure to repair DNA produces a mutation.

The recent publication of the human genome [Link] has already revealed 130 genes whose products participate in DNA repair. More will probably be identified soon.

Agents that Damage DNA

Types of DNA Damage

  1. All four of the bases in DNA (A, T, C, G) can be covalently modified at various positions.
  2. Mismatches of the normal bases because of a failure of proofreading during DNA replication.
  3. Breaks in the backbone.
  4. Crosslinks Covalent linkages can be formed between bases Several chemotherapeutic drugs used against cancers crosslink DNA [Link].

Repairing Damaged Bases

Damaged or inappropriate bases can be repaired by several mechanisms:

Direct Reversal of Base Damage

Perhaps the most frequent cause of point mutations in humans is the spontaneous addition of a methyl group (CH3-) (an example of alkylation) to Cs followed by deamination to a T. Fortunately, most of these changes are repaired by enzymes, called glycosylases, that remove the mismatched T restoring the correct C. This is done without the need to break the DNA backbone (in contrast to the mechanisms of excision repair described below).

Some of the drugs used in cancer chemotherapy ("chemo") also damage DNA by alkylation. Some of the methyl groups can be removed by a protein encoded by our MGMT gene. However, the protein can only do it once, so the removal of each methyl group requires another molecule of protein.

This illustrates a problem with direct reversal mechanisms of DNA repair: they are quite wasteful. Each of the myriad types of chemical alterations to bases requires its own mechanism to correct. What the cell needs are more general mechanisms capable of correcting all sorts of chemical damage with a limited toolbox. This requirement is met by the mechanisms of excision repair.

Base Excision Repair (BER)

The steps and some key players:
  1. removal of the damaged base (estimated to occur some 20,000 times a day in each cell in our body!) by a DNA glycosylase. We have at least 8 genes encoding different DNA glycosylases each enzyme responsible for identifying and removing a specific kind of base damage.
  2. removal of its deoxyribose phosphate in the backbone, producing a gap. We have two genes encoding enzymes with this function.
  3. replacement with the correct nucleotide. This relies on DNA polymerase beta, one of at least 11 DNA polymerases encoded by our genes.
  4. ligation of the break in the strand. Two enzymes are known that can do this; both require ATP to provide the needed energy.

Nucleotide Excision Repair (NER)

NER differs from BER in several ways. The steps and some key players:
  1. The damage is recognized by one or more protein factors that assemble at the location.
  2. The DNA is unwound producing a "bubble". The enzyme system that does this is Transcription Factor IIH, TFIIH, (which also functions in normal transcription).
  3. Cuts are made on both the 3' side and the 5' side of the damaged area so the tract containing the damage can be removed.
  4. A fresh burst of DNA synthesis — using the intact (opposite) strand as a template — fills in the correct nucleotides. The DNA polymerases responsible are designated polymerase delta and epsilon.
  5. A DNA ligase covalently inserts the fresh piece into the backbone.

Xeroderma Pigmentosum (XP)

XP is a rare inherited disease of humans which, among other things, predisposes the patient to It turns out that XP can be caused by mutations in any one of several genes — all of which have roles to play in NER. Some of them:

Transcription-Coupled NER

Nucleotide-excision repair proceeds most rapidly This enhancement of NER involves XPB, XPD, and several other gene products. The genes for two of them are designated CSA and CSB (mutations in them cause an inherited disorder called Cockayne's syndrome).

The CSB product associates in the nucleus with RNA polymerase II, the enzyme responsible for synthesizing messenger RNA (mRNA), providing a molecular link between transcription and repair.

One plausible scenario: If RNA polymerase II, tracking along the template (antisense) strand), encounters a damaged base, it can recruit other proteins, e.g., the CSA and CSB proteins, to make a quick fix before it moves on to complete transcription of the gene.

Mismatch Repair (MMR)

Mismatch repair deals with correcting mismatches of the normal bases; that is, failures to maintain normal Watson-Crick base pairing (A•T, C•G)

It can enlist the aid of enzymes involved in both base-excision repair (BER) and nucleotide-excision repair (NER) as well as using enzymes specialized for this function.

Mutations in either of these genes predisposes the person to an inherited form of colon cancer. So these genes qualify as tumor suppressor genes.

Question: How does the MMR system know which is the incorrect nucleotide? In E. coli, certain adenines become methylated shortly after the new strand of DNA has been synthesized. The MMR system works more rapidly, and if it detects a mismatch, it assumes that the nucleotide on the already-methylated (parental) strand is the correct one and removes the nucleotide on the freshly-synthesized daughter strand. How such recognition occurs in mammals is not yet known.

Synthesis of the repair patch is done by DNA polymerase delta.

Cells also use the MMR system to enhance the fidelity of recombination; i.e., assure that only homologous regions of two DNA molecules pair up to cross over and recombine segments (e.g., in meiosis).

Repairing Strand Breaks

Ionizing radiation and certain chemicals can produce both single-strand breaks (SSBs) and double-strand breaks (DSBs) in the DNA backbone.

Single-Strand Breaks (SSBs)

Breaks in a single strand of the DNA molecule are repaired using the same enzyme systems that are used in Base-Excision Repair (BER).

Double-Strand Breaks (DSBs)

There are two mechanisms by which the cell attempts to repair a complete break in a DNA molecule:

Meiosis also involves DSBs

Recombination between homologous chromosomes in meiosis I also involves the formation of DSBs and their repair. So it is not surprising that this process uses the same enzymes. [Link to a discussion with diagrams showing how this may work.]

Meiosis I with the alignment of homologous sequences provides a mechanism for repairing damaged DNA; that is, mutations. in fact, many biologists feel that the main function of sex is to provide this mechanism for maintaining the integrity of the genome. [More]

However, most of the genes on the human Y chromosome have no counterpart on the X chromosome, and thus cannot benefit from this repair mechanism. They seem to solve this problem by having multiple copies of the same gene — oriented in opposite directions. Looping the intervening DNA brings the duplicates together and allowing repair by homologous recombination.

Gene Conversion

If the sequence used as a template for repairing a gene by homologous recombination differs slightly from the gene needing repair; that is, is an allele, the repaired gene will acquire the donor sequence. This nonreciprocal transfer of genetic information is called gene conversion.

The donor of the new gene sequence may be: Gene conversion during meiosis alters the normal mendelian ratios. Normally, meiosis in a heterozygous (A,a) parent will produce gametes or spores in a 1:1 ratio; e.g., 50% A; 50% a. However, if gene conversion has occurred, other ratios will appear. If, for example, an A allele donates its sequence as it repairs a damaged a allele, the repaired gene will become A, and the ratio will be 75% A; 25% a.

Cancer Chemotherapy

The table lists (by trade name as well as generic name) some of the anticancer drugs that specifically target DNA.

6-mercaptopurinePurinethol®purine analog. One effect: substitutes for G, inducing abortive MMR and strand breaks
GemcitabineGemzar®pyrimidine analog substitutes for C blocking strand elongation
Cyclophosphamide Cytoxan® alkylating agents; form interstrand and/or intrastrand crosslinks
Melphalan Alkeran®
Busulfan Myleran®
Chlorambucil Leukeran®
Mitomycin Mutamycin®
Cisplatin Platinol® forms crosslinks
Bleomycin Blenoxane® cuts DNA strands between GT or GC
Irinotecan Camptosar® inhibit the proper functioning of enzymes (topoisomerases) needed to unwind DNA for replication and transcription
Mitoxantrone Novantrone®
Doxorubicin Adriamycin®
Dactinomycin Cosmegen® inserts into the double helix preventing its unwinding

Sadly,

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17 April 2014